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Systematic Variation in Gene Expression Patterns in Human Cancer Cell Lines
Ross, D.T., Scherf, U., Eisen, M.B., Perou, C.M., Spellman, P.,
Iyer, V., Jeffrey, S.S., Van de Rijn, M., Waltham, M., Pergamenschikov, A., Lee, J.C.F.,
Lashkari, D., Shalon, D., Myers, T.G., Weinstein, J.N., Botstein, D., and Brown, P.O.
Nature Genetics, 2000 March, 24(3):227-234
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Abstract: We used cDNA
microarrays to explore the variation in expression of approximately 8000 unique genes
among the 60 cell lines used in the National Cancer Institute's screen for anti-cancer
drugs. Classification of the cell lines based solely on the observed patterns of gene
expression revealed a striking correspondence to the ostensible origins of the tumours
from which the cell lines were derived. The consistent relationship between the gene
expression patterns to the tissue of origin enabled detection of outliers whose previous
classification appeared incorrect. Specific features of the gene expression patterns were
correlated with other known properties of the cell lines such as their doubling time in
culture while others appeared to be related to physiological processes such as drug
metabolism or the interferon response. Comparison of gene expression patterns in the cell
lines to that in normal breast tissue and breast tumor specimens revealed features of the
expression patterns in the tumours that had recognizable counterparts in different cell
lines reflecting the tumour, stromal, and inflammatory components of the cancer specimens.
Taken together, these results provided a novel molecular characterization of this
important group of human cell lines and their relationships to tumors in vivo.
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